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weird bacteria update

Trond Erik Vee Aune trondaun at biotech.REMOVETHISBEFOREREPLYING.ntnu.no
Thu Mar 20 02:32:19 EST 2003

Thanks for all your suggestions, Judy!

JEDilworth wrote:
> http://www.wisc.edu/fri/annrpt/bcer2001.pdf
> This is an article in pdf format regarding Bacillus species that can 
> swarm. This may possibly be what you're dealing with, as opposed to a 
> gram negative rod.

It does stain the same color as E.coli. It may not be completely clear 
from my pictures, but this is caused by bad equipment. So I find it hard 
to believe it's a Gram-positive strain.

> It's very difficult to see colony morphology with all your colonies up 
> in the inoculum area. 

I couldn't do this since my object was to contain the growth in the 
small area behind the red line. The point with this picture was to show 
that I have managed to keep the bacteria from spreading.

After this picture was taken I did it once more on a bigger area, and 
obtained distinct, single colonies. They all looked the same.

> Also, your gram stain looks more like a gram positive rod, if it is 
> indeed a gram stain, as opposed to a gram negative. Are you sure you 
> have decolorized adequately?

I have to admit I don't have a lot experience with Gram staining, but I 
think I got it right ;) Anyway, as soon as I get the results from 16s 
sequencing we will now for sure.

Trond Erik

Trond Erik Vee Aune
Department of Biotechnology
Norwegian University of Science and Technology
Email: trondaun at biotech.ntnu.no

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