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wierd bacteria update

Scott Coutts scott.coutts at med.monash.edu.au
Mon Mar 17 17:03:58 EST 2003

Yeah, I agree. It's hard to tell from the pics, since they're a little 
too out of focus and it's hard to see the colour properly.

Trond: Make sure you streak out your bug fully to obtain seperate 
colonies before you do the sequencing, of course. Rather than streaking 
just a part of the plate, I mean.

Good luck!

Subhash Kulkarni wrote:
> i really cant see the novel bacteria having pink color...... with the 
> morphology and the colony type, i would suspect it to be a kind of an 
> actinomycete or a bacillus......though on second thoughts since u press 
> the thing that the bacteria does look pink, lactobacter cant be excluded 
> out....but these remain guesses and of course nothing can be said before 
> u try out the sequencing
> Trond Erik Vee Aune wrote:
>> Hi
>> I've now done some more work on the bacteria.
>> The first picture shows a LB plate with 6% agar:
>> http://www.biotech.ntnu.no/~trondaun/xbug/6%20percent%20agar.JPG
>> I spread the colonies on the area indicated with the red line. As you 
>> can see the higher concentration of agar keep the bacteria from 
>> spreading almost totally. A few colonies are growing just over the 
>> line, but it's much less than on 2% agar. Also the colonies are 
>> forming close to eachother, like continous growth, on 2% agar they 
>> would form discrete colonies. The growth seem homogenous and it's not 
>> possible to see different fenotypes.
>> What I've done with this plate after this picture was taken, is to 
>> spread out some other bacteria perpendicular to the x-bug growth to 
>> look at the inhibition pattern.
>> The next picture shows the glass plates after Gram staining:
>> http://www.biotech.ntnu.no/~trondaun/xbug/gram.JPG
>> I used E.coli for control. As you can see I had more cells with 
>> E.coli, but  the color is the same. It looks as my bug x is Gram 
>> negative.
>> I took a few pictures of the bug through the microscope as suggested 
>> earlier, it was difficult to focus, but it should give some hints to 
>> the stain and the morphology of the bacteria. All pictures are with 
>> 100x magnifying and with oil on the lens.
>> The first picture is of the E.coli:
>> http://www.biotech.ntnu.no/~trondaun/xbug/ecoli.JPG
>> The next shows the x bug:
>> http://www.biotech.ntnu.no/~trondaun/xbug/x1.JPG
>> It's a little unfocused but it should be possible to see that it is 
>> longer than E.coli. Much more stretched out. It also has the same 
>> color as E.coli after staining.
>> The last picture shows the same:
>> http://www.biotech.ntnu.no/~trondaun/xbug/x2.JPG
>> Tomorrow I'll order the primers needed to do the 16s sequencing, and 
>> hopefully I'll have some pictures of the inhibition pattern to show 
>> you then.
>> Trond Erik

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