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Any advice on growing stubborn yeast again?

Warren Place wrplace at mailbox.ucdavis.edu
Mon Mar 17 16:23:02 EST 2003


Does this medium contain yeast extract?  If not, YPD (yeast extract +
peptone + dextrose) might give better results.

On 11 Mar 2003 iwbt28 at sun.ac.za wrote:

> I cannot seem to make very old (about a year) yeast cultures on Yeast Malt (YM)
> media to grow again.
> They are mostly industrial strains of S.cerevisiae and a few wild yeast too.
> I've tried streaking out on
> YM agar media as well as liquid cultures of YM (pH adjusted to 5.5) media
> supplemented with vitamins too.
>
> Any ideas will be greatly appreciated!
>
> http://biowww.net/mynews/tree.php?group_name=bionet_microbiology&begin=0
>

Warren Place




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