Does this medium contain yeast extract? If not, YPD (yeast extract +
peptone + dextrose) might give better results.
On 11 Mar 2003 iwbt28 at sun.ac.za wrote:
> I cannot seem to make very old (about a year) yeast cultures on Yeast Malt (YM)
> media to grow again.
> They are mostly industrial strains of S.cerevisiae and a few wild yeast too.
> I've tried streaking out on
> YM agar media as well as liquid cultures of YM (pH adjusted to 5.5) media
> supplemented with vitamins too.
>> Any ideas will be greatly appreciated!