i really cant see the novel bacteria having pink color...... with the
morphology and the colony type, i would suspect it to be a kind of an
actinomycete or a bacillus......though on second thoughts since u press
the thing that the bacteria does look pink, lactobacter cant be excluded
out....but these remain guesses and of course nothing can be said before
u try out the sequencing
Trond Erik Vee Aune wrote:
> Hi
>> I've now done some more work on the bacteria.
>> The first picture shows a LB plate with 6% agar:
>http://www.biotech.ntnu.no/~trondaun/xbug/6%20percent%20agar.JPG> I spread the colonies on the area indicated with the red line. As you
> can see the higher concentration of agar keep the bacteria from
> spreading almost totally. A few colonies are growing just over the line,
> but it's much less than on 2% agar. Also the colonies are forming close
> to eachother, like continous growth, on 2% agar they would form discrete
> colonies. The growth seem homogenous and it's not possible to see
> different fenotypes.
>> What I've done with this plate after this picture was taken, is to
> spread out some other bacteria perpendicular to the x-bug growth to look
> at the inhibition pattern.
>> The next picture shows the glass plates after Gram staining:
>http://www.biotech.ntnu.no/~trondaun/xbug/gram.JPG> I used E.coli for control. As you can see I had more cells with E.coli,
> but the color is the same. It looks as my bug x is Gram negative.
>> I took a few pictures of the bug through the microscope as suggested
> earlier, it was difficult to focus, but it should give some hints to the
> stain and the morphology of the bacteria. All pictures are with 100x
> magnifying and with oil on the lens.
>> The first picture is of the E.coli:
>http://www.biotech.ntnu.no/~trondaun/xbug/ecoli.JPG> The next shows the x bug:
>http://www.biotech.ntnu.no/~trondaun/xbug/x1.JPG> It's a little unfocused but it should be possible to see that it is
> longer than E.coli. Much more stretched out. It also has the same color
> as E.coli after staining.
> The last picture shows the same:
>http://www.biotech.ntnu.no/~trondaun/xbug/x2.JPG>> Tomorrow I'll order the primers needed to do the 16s sequencing, and
> hopefully I'll have some pictures of the inhibition pattern to show you
> then.
>> Trond Erik
>
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