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weird bacteria - Update

Trond Erik Vee Aune trondaun at biotech.NOSPAM.ntnu.no
Tue Mar 11 12:15:03 EST 2003

DenniS wrote:
> His picture looks like it is a pure plate more than a streak plate.

As I told in an earlier posting it _is_ a streak plate, but the colonies 
do not form continously, they sort of just pop up somewhere along where 
I've streaked. And they also pop up away from where I originally 
streaked because they are swarming. After a few days they have covered 
the whole plate, but they still do not seem to like growing close to 
eachother, so it is only sporadic colonies all over the plate.

The question now is whether this strange growth is caused by one strain 
or more.

Trond Erik

>  but the
> growth on some of the colonies look odd? 

They have an irregular border. And in some cases they develop in an 
spiral (you can see this on some of the closest colonies). But I 
wouldn't say that there is any reason to suspect multiple strains 
because of this, the colonies seem fairly homogenous to me.

> Judy does the colonies look like a
> GNR to you?

What's this then?

Trond Erik

> DenniS
> "JEDilworth" <bactitech at nospamhortonsbay.com> wrote in message
> news:3E6CBF70.90706 at nospamhortonsbay.com...
>>It sounds like you're doing micro without a loop or wire to pick
>>colonies or something to flame your loop in between touching colonies.
>>After you make your 6% agar (or use some differential media as I
>>described in another posting) you need to streak for isolation. If you
>>obtain two colony morphologies, each colony is a pure culture. You need
>>to flame your loop, pick one colony and streak it to another plate. You
>>then need to flame your loop again, pick the second colony type, plate
>>to a separate plate and streak for isolation. Then flame again.
>>This is basic microbiology. If you're using bacteria, even in a genetics
>>laboratory, I don't understand how you can do it without all of the
>>basic equipment that you need. The ONLY way to separate bacteria is
>>streak for isolation, using solid media. You put your inoculum down on
>>about 1/3 of the plate. Then you turn the plate a little and streak out
>>from this area. You then flame, and then streak another quadrant, then
>>turn and streak the last quadrant. You then incubate the plate upside
>>down, at least overnight, and look for different colony morphology.
>>A visit to your local clinical micro lab will help you immensely! We do
>>this EVERY DAY. Gram negative rods don't look THAT different from each
>>other upon staining, at least with Gram's stain. Some are smaller and
>>thinner or thicker, but that doesn't help you separate them out.
>>Judy Dilworth, M.T. (ASCP)

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