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weird bacteria - Update

DenniS grtmilenko at mindspring.com
Tue Mar 11 07:40:52 EST 2003


His picture looks like it is a pure plate more than a streak plate.  but the
growth on some of the colonies look odd?  Judy does the colonies look like a
GNR to you?

DenniS


"JEDilworth" <bactitech at nospamhortonsbay.com> wrote in message
news:3E6CBF70.90706 at nospamhortonsbay.com...
> It sounds like you're doing micro without a loop or wire to pick
> colonies or something to flame your loop in between touching colonies.
>
> After you make your 6% agar (or use some differential media as I
> described in another posting) you need to streak for isolation. If you
> obtain two colony morphologies, each colony is a pure culture. You need
> to flame your loop, pick one colony and streak it to another plate. You
> then need to flame your loop again, pick the second colony type, plate
> to a separate plate and streak for isolation. Then flame again.
>
> This is basic microbiology. If you're using bacteria, even in a genetics
> laboratory, I don't understand how you can do it without all of the
> basic equipment that you need. The ONLY way to separate bacteria is
> streak for isolation, using solid media. You put your inoculum down on
> about 1/3 of the plate. Then you turn the plate a little and streak out
> from this area. You then flame, and then streak another quadrant, then
> turn and streak the last quadrant. You then incubate the plate upside
> down, at least overnight, and look for different colony morphology.
>
> A visit to your local clinical micro lab will help you immensely! We do
> this EVERY DAY. Gram negative rods don't look THAT different from each
> other upon staining, at least with Gram's stain. Some are smaller and
> thinner or thicker, but that doesn't help you separate them out.
>
> Judy Dilworth, M.T. (ASCP)
> Microbiology
>





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