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weird bacteria - Update

Scott J. Coutts scott.coutts at med.monash.edu.au
Fri Mar 7 21:02:10 EST 2003


Anne Thomas wrote:
> "Scott J. Coutts" <scott.coutts at med.monash.edu.au> wrote in message
> news:3E6883CC.9040508 at med.monash.edu.au...
> 
>>
>>Trond Erik Vee Aune wrote:
>>
>>>>I'd recommend the 6% agar trick first of all. It will also make those
>>>>colonies smaller, too. Spread them onto a plate.
>>>>
>>>
>>>But how with this help me separate them? It'll only prevent the swarmers
>>>from spreading. I'll still have the two strains together.
>>>
>>
>>Yup, that's the idea. I assume you know how to streak a plate to obtain
>>single colonies? I.e. streak an initial inoculum over  a section of the
>>plate, flame your loop, do a set of streakes coming from the first
>>incolulation point, flame the loop, set of streaks from first set of
>>streaks, flame etc etc.
>>
> 
> 
> 
> Doesn't it make you wish you could actually be there and show Trond what we
> mean about separating colonies by streaking them out?
> 

Hehe yes, it's kind of hard to describe sometimes! Actually, I was half 
asleep when I wrote that, so it's not a very thorough description (:

> Hopefully Trond, if you have 2 organisms there, the colonies may look
> different on 6% and then you could subculture them again separately.
> 
> Good Luck!
> Annie :o)
> 




-- 
Scott J. Coutts
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