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weird bacteria - Update

Anne Thomas annie_t at ntlworld.yeah_right.com
Fri Mar 7 19:02:11 EST 2003


"Scott J. Coutts" <scott.coutts at med.monash.edu.au> wrote in message
news:3E6883CC.9040508 at med.monash.edu.au...
>
>
> Trond Erik Vee Aune wrote:
> >> I'd recommend the 6% agar trick first of all. It will also make those
> >> colonies smaller, too. Spread them onto a plate.
> >>
> >
> > But how with this help me separate them? It'll only prevent the swarmers
> > from spreading. I'll still have the two strains together.
> >
>
> Yup, that's the idea. I assume you know how to streak a plate to obtain
> single colonies? I.e. streak an initial inoculum over  a section of the
> plate, flame your loop, do a set of streakes coming from the first
> incolulation point, flame the loop, set of streaks from first set of
> streaks, flame etc etc.
>


Doesn't it make you wish you could actually be there and show Trond what we
mean about separating colonies by streaking them out?

Hopefully Trond, if you have 2 organisms there, the colonies may look
different on 6% and then you could subculture them again separately.

Good Luck!

Annie :o)







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