"Scott J. Coutts" <scott.coutts at med.monash.edu.au> wrote in message
news:3E6883CC.9040508 at med.monash.edu.au...
>>> Trond Erik Vee Aune wrote:
> >> I'd recommend the 6% agar trick first of all. It will also make those
> >> colonies smaller, too. Spread them onto a plate.
> > But how with this help me separate them? It'll only prevent the swarmers
> > from spreading. I'll still have the two strains together.
>> Yup, that's the idea. I assume you know how to streak a plate to obtain
> single colonies? I.e. streak an initial inoculum over a section of the
> plate, flame your loop, do a set of streakes coming from the first
> incolulation point, flame the loop, set of streaks from first set of
> streaks, flame etc etc.
Doesn't it make you wish you could actually be there and show Trond what we
mean about separating colonies by streaking them out?
Hopefully Trond, if you have 2 organisms there, the colonies may look
different on 6% and then you could subculture them again separately.