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weird bacteria - Update

Scott J. Coutts scott.coutts at med.monash.edu.au
Fri Mar 7 06:31:16 EST 2003

Trond Erik Vee Aune wrote:
>> I'd recommend the 6% agar trick first of all. It will also make those 
>> colonies smaller, too. Spread them onto a plate. 
> But how with this help me separate them? It'll only prevent the swarmers 
> from spreading. I'll still have the two strains together.

Yup, that's the idea. I assume you know how to streak a plate to obtain 
single colonies? I.e. streak an initial inoculum over  a section of the 
plate, flame your loop, do a set of streakes coming from the first 
incolulation point, flame the loop, set of streaks from first set of 
streaks, flame etc etc.

Otherwise, do a set of serial dilutions of your broth, and plate out 
100uL of the dilutions on your plates. Some of them should end up with 
very few single colonies.

Scott J. Coutts
  Bacterial Pathogenesis Research Group		
  Department of Microbiology			Ph  [+61 3 9905 4838]
  PO Box 53					Fax [+61 3 9905 4811]	
  Monash University, 3800, Austrlalia

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