Scott J. Coutts wrote:
> Did you see more than one cell shape in the stain? Could you post a pic
> of it from the microscope on the web (if you have the facilities)?
I could only see one shape. I don't have a microscope with a camera
attached. But I'll check with other departments.
> > I'm still eager to do 16s sequencing, but first I need to separate
> > them if there's more than one strain. Do you microbiologists have any
> > idea to how I could separate them?
>> I'd recommend the 6% agar trick first of all. It will also make those
> colonies smaller, too. Spread them onto a plate.
But how with this help me separate them? It'll only prevent the swarmers
from spreading. I'll still have the two strains together.
> I'm still not convinced
> that you have two types. Do you have a dark field or phase contrast
> microscope? (probably not if you're a genetics lab). You may see a
> difference in the motility if you can look at them directly.
I don't know, I'll have to check it up.
Trond Erik Vee Aune
Department of Biotechnology, NTNU
Sem Selandsvei 6/8
Tlf: +0047 73551386
Email: trondaun at biotech.ntnu.nohttp://www.biotech.ntnu.no/molgen