Oh, wait, did you say you inoculated from a site on the plate with no
apparent colonies? In that case, I'd say definitely there is two types.
If you could post a pic of the stain, that'd probably help, maybe using
the 40x and 100x (with oil) objectives if possible.
Scott J. Coutts wrote:
> Trond Erik Vee Aune wrote:
> >
> > Scott J. Coutts wrote:
> >
> >> I agree that it should be stained.
> >
> >
> > It took me some time to do the staining, but now it's done. I
> > compared it with E.coli. It looks to be gram negative.
> >
> > I've also tried to separate the "swarmer" from the "colony former" by
> > inoculating from an area without any obvious colony growth and from
> > a colony, but without success. In both cases I get the same growth as
> > described earlier. So maybe it is one strain after all?
> >
>> Did you see more than one cell shape in the stain? Could you post a pic
> of it from the microscope on the web (if you have the facilities)?
>> >
> > I'm still eager to do 16s sequencing, but first I need to separate
> > them if there's more than one strain. Do you microbiologists have any
> > idea to how I could separate them?
> >
>> I'd recommend the 6% agar trick first of all. It will also make those
> colonies smaller, too. Spread them onto a plate. I'm still not convinced
> that you have two types. Do you have a dark field or phase contrast
> microscope? (probably not if you're a genetics lab). You may see a
> difference in the motility if you can look at them directly.
>> >
> > Actually a 16s sequencing would tell me if I have more than one
> > genotype. But it would be better to establish this before ordering
> > the primers. At least if there's an easy way to do this.
> >
>> Well, even if you separate them (assuming there is actually two to be
> separated), you're still going to need to order the primers... why not
> order them anyhow (:
>> >
> > Thanks again for all your helpful suggestions!
> >
> > Trond Erik
> >
>>
--
Scott J. Coutts
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