Trond Erik Vee Aune <trondaun at biotech.REMOVETHISBEFOREREPLYING.ntnu.no> writes:
> other different bacteria, I streaked it onto a new plate. For some
> reason it did not come up as a continous smear, but as distinct
> colonies, even where I had put a large amount of colony material. And
> more surprising, it even came up at places where I had not streaked. I
> tried it again, and this time I shook the needle before streaking to
> try to remove possible spores. When streaking, I made sure to keep the
> needle close to the surface of the agar. Despite of all these efforts,
> new colonies grew up up to 1,5 cm away from the area where I had put
> the colony material. And still no continous growth, almost like they
> are inhibiting themselves.
I presume you tried culturing one of your plates without streaking
anything on it... If not, and you get colonies on it, then this
sounds like your autoclave needs fixing.