Artem Evdokimov wrote:
> Hi,
>> Congratulations on the ID. Remind us, did your bug have flagella ? P. alvei
> is supposed to be flagellated... It is a common beehive parasite if memory
> serves right, it can be found on dead bee larvae. It sporulates with spores
> positioned in almost ordered rows. Sorry to hear that your bug isn't brand
> new...
Yes, it was highly motile and swarmed in a very fascinating way. That's
why I decided to identify it. I looked for spores in the microscope, but
couldn't see any, so maybe the cells were in the wrong growth phase.
Trond Erik
>> Artem
>>> "Trond Erik Vee Aune" <trondaun at biotech.REMOVETHISBEFOREREPLYING.ntnu.no>
> wrote in message
> news:3EAD0F42.2000002 at biotech.REMOVETHISBEFOREREPLYING.ntnu.no...>>>Hi all,
>>>>I just received the sequencing data of the "wierd bacterium" we've been
>>talking about earlier. Unfortunately, the sequence was not very good,
>>but I did get what I thnik is convincing results when blasting. I have
>>pasted the sequence so that you can check it out yourselves if you're
>>interested.
>>>>Anyway, I got very good match against Paenibacillus alvei. According to
>>web-resources this genus do produce different types of antibiotics as
>>well as being able to swarm in different ways
>>(http://ijs.sgmjournals.org/cgi/reprint/49/1/239.pdf). The strange thing
>>is that it is not a gram negative bug as I thought after staining, but
>>one of you microbiologists suggested that I was a Bacillus strain, so I
>>guess we geneticists are much better at sequencing than staining ;)
>>Anyway, I'm pretty pleased that I finally got an identification, but of
>>course a little disappointed that it wasn't a new bacterium
>>>>Forward sequence:
>>>> GGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACTTGATGGAGTGCTTGCACTCCTGATGGTTAG
> CGGC
>> GGACGGGTGAGTAACACGTACGTAACCTGCCCATAAGACTGGGATAACCCACNGAAACGTGAGCTANTACCANATN
> GGCN
>> TTTTCCTCGCATGAGGGAAATNAGAAAGGCGGATCAATCTGTCNCTTATGGATGGACCTGCGGNGCATTATCTAGT
> TGGT
>> GAGGTAACGGCTNACCAAGGCGACGATGCGTNCCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGANACACGG
> TCCC
>> AGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTNTGACGGAGCAACGCCGTCGTGAGTG
> ATNA
>> ANGTTTTCNGATCGTAAAGCTCTGNTGCCANGGAANAACGCCTAGGAGAGTNACTNCTCTTAGGGNGACGGNNCCT
> GAGA
>> AGAAAGCCCCTNCTAACTACNTGCCANCAGCCGNGGTANTACGTANGGGGCGAGCGTNNNCCANANTTATTNGGCT
> TAAA
>> GCGCGCNCAGGCAGNAATNTAAGTTGGGTGTTTAAACCTAGGGCTCAACCTTGGGNCGCATNCAAAACTGCATANC
> TNGA
>>>NTACAGAAAGAGGAAAGNGGAATNCCNCGTGTTCCNGNGAAATGCGTANAGATNTCNAGGAACACNATTGN
>>>>>>Reverse sequence:
>>>> GGNACCCCAATCATCTACCCCACCTTCGGCGGCTGGCNCCTTGCGGTTACCTCACCGACTTCGGGTGTTGTAAACT
> CTCG
>> TGGTGTGACGGGCGGNGTGTACAAGACCCGGGAACGTATTCACCGCTGGCATGCTGATCCGCGATTACTAGCAATT
> CCGA
>> CTTCATGCAGGCGAGTTGCAGCCTGCAATCCGAACTGAGACTGGCTTTTTAGGATTGGCTNCACCTCGCGGNTTCG
> CTTC
>> CCGATGTACCAGCCATTGTAGNACGTGNGTAGCCCAGGTCATAAGGGGCATGATGATTNGANNTNATNCTCACCTT
> CCTC
>> CGGTACTGTCACCGGCAGGCACTNTAGAGTGNCCAACTGAATGCTGGCAANTAAAGANAAAGGTTGCGCTCGTGAG
> CGGG
>> ACTTAACCCAACATTTNACAACANNAGCTGACGACAACCATNCACNACCNGANTANAAATGTCCNNANAGAAAGCC
> CTAT
>> CTCTAGAACGNNCCTTCATATNTCAAGACCTGGTAAGGNTNTTNCCGTTGCTTCNAATTTNACCCCATACTTCACT
> GCNT
>> TTNCCNGCNCCCCGCNCGTTCCTTTTAGNTTTCAAGTCTTTNCGCACCGTGCTTCCCCACGCGGAATGCTTTAATG
> ANTT
>> ACTTTCTNCCCCAAGGGAAATAGAAAACCCGNAATACCNAGTCTTNCTTGNTATACCNGCTNGGGCTTNCGGGGNA
> TCNA
>> ATNCCGTTTGCTCCNNCTTCCTTTCTCCNCNTNNCNNNTNGNTNCCNCCCNANAGGCNNCTTCCCCNATGGGATNC
> CTCN
>> CNTNCTCCNCTAGNNCCGNAAACTNGGTAAGAAGATNNTTTTTCTNNNAACNAGTAANTCTAAACGNACCACNNGG
> GATN
>> GGNAAAATAANTAATANANANAGGNACTAGACCAAGNTNNNGAGATNNTACCNAAAANNAANTTAGGGATNAGANA
> NAGN
>>>NTTTTANACTACT
>>>>If you have any more information about this bacterium, please tell me,
>>I'm interested :)
>>>>Regards,
>>Trond Erik
>>>>>>--
>>Trond Erik Vee Aune
>>Department of Biotechnology
>>Norwegian University of Science and Technology
>>Email: trondaun at biotech.ntnu.no>>http://www.REMOVETHISBEFOREREPLYING!!biotech.ntnu.no/molgen>>>>>