Subhash Kulkarni wrote:
> good to see the results at last, the sequencing does suggest that as
> predicted its not gram negative and does indeed belong to bacillus. i
> suggested that it is a bacillus type rather than a gram negative, and
> dear geneticist, it does confirm that experience can save u some amount
> of work when u have foresight to do so...
:) Well, even if i did get a convincing stain I would have done the
sequencing, I would never settle for just stain information.
> cheers with the results
> PS: whats the thing with these bacteria being from ganges???
Just a friend of mine who brought some water sample fra Ganges,
something I've been playing around with on my spare time. Well, I
haven't been "playing around" with the water considering all the
potential harmful bugs that are probably in there ;)...But I have done
som tests to establish what kind of microorganisms is in the water.
>> Trond Erik Vee Aune wrote:
>>> Hi all,
>>>> I just received the sequencing data of the "wierd bacterium" we've
>> been talking about earlier. Unfortunately, the sequence was not very
>> good, but I did get what I thnik is convincing results when blasting.
>> I have pasted the sequence so that you can check it out yourselves if
>> you're interested.
>>>> Anyway, I got very good match against Paenibacillus alvei. According
>> to web-resources this genus do produce different types of antibiotics
>> as well as being able to swarm in different ways
>> (http://ijs.sgmjournals.org/cgi/reprint/49/1/239.pdf). The strange
>> thing is that it is not a gram negative bug as I thought after
>> staining, but one of you microbiologists suggested that I was a
>> Bacillus strain, so I guess we geneticists are much better at
>> sequencing than staining ;) Anyway, I'm pretty pleased that I finally
>> got an identification, but of course a little disappointed that it
>> wasn't a new bacterium
>>>> Forward sequence:
>>>>>> Reverse sequence:
>>>> If you have any more information about this bacterium, please tell me,
>> I'm interested :)
>> Trond Erik
Trond Erik Vee Aune
Department of Biotechnology
Norwegian University of Science and Technology
Email: trondaun at biotech.ntnu.nohttp://www.REMOVETHISBEFOREREPLYING!!biotech.ntnu.no/molgen