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HELP::Unknown Bacteria Identify!!

Subhash Kulkarni Subhash.Kulkarni at nospam.ie-zea.unil.ch
Mon Apr 28 04:32:28 EST 2003


a better way to go in would be to sequence the 16S rDNA gene, 
biochemical characterization are on their way to become obsolete

lgl_free at yahoo.com wrote:
> Thank first! I know that there are many Biology Nu Ren here in this board.  
> 
> Please help!  
> 
> I have an unknow bacteria. I did a lot of test on it, still don't know which  
> 
> one it belongs to.  
> 
>   
> 
> Gram positive coci, short chain,nonmotile, catalase negative,  
> 
> Growth at   
> 
> ph9.6 +  
> 
> NaCl 6.5% + (incubate for about one week)  
> 
> Sheep Blood Agar:  
> 
> Alpha hemolysis -  
> 
> Beta hemolysis -  
> 
> Gamma hemolysis +  
> 
> Aginie hydrolysis: +  
> 
> Esculin hydolysis: +  
> 
> Starch hydrolysis: -  
> 
> Hippurate hydrolysis: -  
> 
> D-Xylose: +(incubate for about one week)  
> 
> L-Rhamnose: -  
> 
> Sucrose: +  
> 
> Raffinose: -  
> 
> Glycerol: +  
> 
> Adonitol: -  
> 
> Sorbitol: +  
> 
> Mannitol: +  
> 
> Inulin: -  
> 
> Trehalose: +  
> 
> Beta-Galactosidase: +  
> 
> CAMP: -  
> 
> SXT sensitivity: R  
> 
> Bacitracin Susceptibility: -  
> 
>   
> 
> I suspect this organism is Enterococus. faecalis(70% confidence), but still  
> 
> not sure because it should be + in Hippurate hydrolysis test, d in alpha  
> 
> hemolysis,and does not ferment D-Xylose. I also can't cross out the  
> 
> possibility of Streptococus. porcinus.   
> 
> Please let me know what's your idea. Thanks very much. 
> 
> 
> 
> http://biowww.net/mynews/tree.php?group_name=bionet_microbiology&begin=0




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