a better way to go in would be to sequence the 16S rDNA gene,
biochemical characterization are on their way to become obsolete
lgl_free at yahoo.com wrote:
> Thank first! I know that there are many Biology Nu Ren here in this board.
>> Please help!
>> I have an unknow bacteria. I did a lot of test on it, still don't know which
>> one it belongs to.
>>>> Gram positive coci, short chain,nonmotile, catalase negative,
>> Growth at
>> ph9.6 +
>> NaCl 6.5% + (incubate for about one week)
>> Sheep Blood Agar:
>> Alpha hemolysis -
>> Beta hemolysis -
>> Gamma hemolysis +
>> Aginie hydrolysis: +
>> Esculin hydolysis: +
>> Starch hydrolysis: -
>> Hippurate hydrolysis: -
>> D-Xylose: +(incubate for about one week)
>> L-Rhamnose: -
>> Sucrose: +
>> Raffinose: -
>> Glycerol: +
>> Adonitol: -
>> Sorbitol: +
>> Mannitol: +
>> Inulin: -
>> Trehalose: +
>> Beta-Galactosidase: +
>> CAMP: -
>> SXT sensitivity: R
>> Bacitracin Susceptibility: -
>>>> I suspect this organism is Enterococus. faecalis(70% confidence), but still
>> not sure because it should be + in Hippurate hydrolysis test, d in alpha
>> hemolysis,and does not ferment D-Xylose. I also can't cross out the
>> possibility of Streptococus. porcinus.
>> Please let me know what's your idea. Thanks very much.