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wierd bacteria update

Scott J. Coutts scott.coutts at med.monash.edu.au
Thu Apr 3 08:05:00 EST 2003

Hey Trond,

Any news on the 16S sequence?

Trond Erik Vee Aune wrote:
> Hi
> I've now done some more work on the bacteria.
> The first picture shows a LB plate with 6% agar:
> http://www.biotech.ntnu.no/~trondaun/xbug/6%20percent%20agar.JPG
> I spread the colonies on the area indicated with the red line. As you 
> can see the higher concentration of agar keep the bacteria from 
> spreading almost totally. A few colonies are growing just over the line, 
> but it's much less than on 2% agar. Also the colonies are forming close 
> to eachother, like continous growth, on 2% agar they would form discrete 
> colonies. The growth seem homogenous and it's not possible to see 
> different fenotypes.
> What I've done with this plate after this picture was taken, is to 
> spread out some other bacteria perpendicular to the x-bug growth to look 
> at the inhibition pattern.
> The next picture shows the glass plates after Gram staining:
> http://www.biotech.ntnu.no/~trondaun/xbug/gram.JPG
> I used E.coli for control. As you can see I had more cells with E.coli, 
> but  the color is the same. It looks as my bug x is Gram negative.
> I took a few pictures of the bug through the microscope as suggested 
> earlier, it was difficult to focus, but it should give some hints to the 
> stain and the morphology of the bacteria. All pictures are with 100x 
> magnifying and with oil on the lens.
> The first picture is of the E.coli:
> http://www.biotech.ntnu.no/~trondaun/xbug/ecoli.JPG
> The next shows the x bug:
> http://www.biotech.ntnu.no/~trondaun/xbug/x1.JPG
> It's a little unfocused but it should be possible to see that it is 
> longer than E.coli. Much more stretched out. It also has the same color 
> as E.coli after staining.
> The last picture shows the same:
> http://www.biotech.ntnu.no/~trondaun/xbug/x2.JPG
> Tomorrow I'll order the primers needed to do the 16s sequencing, and 
> hopefully I'll have some pictures of the inhibition pattern to show you 
> then.
> Trond Erik

Scott J. Coutts
  Bacterial Pathogenesis Research Group		
  Department of Microbiology			Ph  [+61 3 9905 4838]
  PO Box 53					Fax [+61 3 9905 4811]	
  Monash University, 3800, Australia

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