Historians believe that in newspost
<an595j$c3b$1 at prometheus.acsu.buffalo.edu> on Sat, 28 Sep 2002, John M
Neary <neary at acsu.buffalo.edu> penned the following literary
masterpiece:
>Rudy,
>>We currently use pulsed-field gel electrophoresis (PFGE)to resolve bands of
>genomic DNA cut with a rare-cutting restriction enzyme (usually SmaI). You
>may also want to look into multi-locus sequence typing (MLST), which is a
>little more involved, but less ambiguous and easy to reproduce. A PubMed
>search should provide plenty of info on these techniques.
>>John
>>"badman" <badman at sky.be> wrote in message
>news:ok3cpu03eikppsaqaj8e3atenfqrb28lqo at 4ax.com...>> Can someone help me with a good script for DNA-fingerprinting? I am
>> looking to implemnet the technique in the lab and I am looking for a
>> good procedure. I want to do DNA-fingerprinting for Legionella
>> pneumophila; Can someone help me to find the just inforamtion?
>>>> thnak you
>>>> rudy
>>
RAPD or 16s/23s intergenic region PCR plus RE digestion etc. ?
Must be easier than PFGE :-))
Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
Duncan Clark
GeneSys Ltd.
