Bacterial identification & colony counting from a mixture of 5 different bacteria

JEDilworth bactitech at nospamhortonsbay.com
Fri Sep 13 10:29:51 EST 2002

I understand totally. Thanks for the compliment, BTW.

It's just that I don't want to hang myself speculating about stuff that
I know nothing about. Working in a clinical laboratory is certainly
different than an academic laboratory. Unfortunately we see the multiply
resistant bugs we work with on a daily basis as more and more of a
routine thing.

I had a MRSA (methicillin resistant Staph. aureus) from a non-healing
heel wound the other day that was resistant to every drug on our gram
positive battery but Vancomycin! There were NO zone sizes on the KB
sensitivity other than with Vanco (that means resistant to Clindamycin,
Erythromycin, Ampicillin, Penicillin, Trimethoprim Sulfa, Gentamicin,
Levofloxacin, Ciprofloxacin, Oxacillin, and a couple of others).
Luckily, that was still measuring sensitive. We normally do automated
sensitivities on S. aureus on our Vitek, but the Vitek printout was so
resistant I wanted to double check it. They unfortunately matched.

Acinetobacter calcoaceticus var. anitratus is another bug that is
showing up more and more clinically, especially in respiratory
infections from ICU patients. This is a gram negative rod that is also
very resistant. Strains of this from 10-15 years ago did not used to be
this resistant. It's only in the last couple of years we've noticed the
resistance patterns getting worse.

No, you don't want your students to inadvertently be messing with this

Judy Dilworth, M.T. (ASCP)

Lesley Robertson wrote:
> But I always enjoy (and usually agree with) your stuff! It's just that these
> days, about half my life goes into biosafety legislation (implementing it,
> but also trying to keep it reasonable), so that's the first angle the cames
> to my mind!
> Lesley Robertson
> http://www.beijerinck.bt.tudelft.nl

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