Bacterial identification & colony counting from a mixture of 5 different bacteria

JEDilworth bactitech at nospamhortonsbay.com
Wed Sep 11 17:26:33 EST 2002

You will probably need a blood enriched plate to grow these organisms,
at least from my experience in clinical microbiology. I'm not sure about
the A. naeslundii - it may need something richer like brain heart
infusion with blood. Also, you may need CO2 enrichment. Lactobacillus
species don't grow very fast - not sure about caseii.

Are you doing colony counting or just streaking for isolation?

Judy Dilworth, M.T. (ASCP)

"Haider, Amir A" wrote:
> 1-I  want to mix and grow the following bacteria for one of my projects:
> Streptococcus mutans, Lactobacillus caseii, S. salivarius, S. parasanguis, A. naeslundii .
> 2-After growing them, I need to identify and count the colonies, so that, I can compare the growth rates under the conditions of my experiment.
> Now the question is , what type of agar plates should I use so that I can separate  and  count them?

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