>...if you think about it
> By the time you finish mucking around, it would be quicker to do it
Cannot say about the yeast, but I think many know from own experience or
from reading numerous manuals and could answer without moralizing that
OD=0.5 for LB-grown DH5alpha corresponds to approx. 200 mln cells per ml.
Quicker is always to answer the question that was clearly asked, with
details to follow if anybody still interested.