Geobacillus thermoleovorans - Glyoxylatecycle?

EK khatipovNO-SPAM at NO-SPAMuchicago.edu
Sat Nov 30 01:18:02 EST 2002

Nick is right. You cannot assume anything unless you or someone else tested
it experimentally. The fact that fatty acids degrade to acetyl-CoA does not
mean that the only way to replenish Krebs cycle is through glyoxylate shunt.
There might be alternative pathways. Why don't you measure activities of
isocitrate lyase and malate synthase, key enzymes of the glyoxiyate shunt?
It should not be too difficult. Moreover, do you know whether plant oils are
100% fatty acids? I doubt about that...

"Nicholas Landau" <njl2q at virginia.edu> wrote in message
news:3DE6FB21.67DF9A5F at virginia.edu...
> I cannot imagine that too much has been published on Geobacillus
thermoleovorans.  The species was characterized within the last twenty
years, right?  It may be up to you to discover whether this organism
utilizes some known
> enzyme pathway, or if it uses a novel pathway (novel to us, not to the
> If it is important, I would not rely on the assumption that your organism
uses a known enzymatic pathway in metabolism.  Often such assumptions are
incorrect, even when they seem highly intuitive.  A colleague of mine at the
> University of Maryland had her work set back two years because she assumed
that two strains of closely related Pseudomonas species used the same gene
to facilitate adhesion to plastic.
> I guess that isn't very helpful.   Sorry.  My advice is to get some
imperical evidence for the presence of the enzyme in question if it is
important, unless somebody else has already done that experiment.
> Let us know what you find!
> --Nick Landau
> astrid.gascho at stud.uni-bayreuth.de wrote:
> > Hi!
> >
> > I´m working with Geobacillus thermoleovorans. This organism grows on oil
(olive oil, sunflower oil) as single carbon source.
> >
> > Now I need to know if Geobacillus thermoleovorans then has the enzymes
for the glyoxylatecycle or at least if I can suppose that most of the
Bacteria, which grow on oil as single carbon source generally have these
> >
> > Astrid
> >
> > http://biowww.net/mynews/tree.php?group_name=bionet_microbiology&begin=0

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