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Microbio Techniques

Lorren sillyghostSPAM at lycos.com
Wed May 29 01:17:55 EST 2002


I wish to culture P. acnes. You see, I've been cycled through several
antibiotics over the years and I would like to culture the strains specific
to my skin and determine resistance characteristics. I have access to a
community college lab, though I don't belive they have an anerobic
incubation chamber. Perhaps something could be inprovised... and anaroebic
culture bag or so forth. The college doesen't have the literature resources
that are essential for this project, which is why I am inquiring here.

BTW, I've been looking at Current Protocols in Cell Biology published by
Wiley Publishing
(http://shop.barnesandnoble.com/booksearch/isbnInquiry.asp?userid=6A3JMIXK7Y
&mscssid=8RJ8TPB6GH498LVKVPNCAXCJXAS2FMG4&isbn=0471241059)
and was wondering if this collection of protocols would have what I'm
looking for. Any thoughts?



"John Gentile" <yjgent at cox.net> wrote in message
news:B9147669.2F27%yjgent at cox.net...
> In what context are you doing this? Are you in a well supplied lab or are
> you doing this as a hobby? Do you know exactly what organism you are
> planning on working with? Anaerobes don't respond very well to most
> sensitivity testing. NCCLS standards at one time listed only broth
dilution
> as the standard for anaerobe sensitivities, but now the E-Test and other
MIC
> methods work well.
>
> Basically you need the appropriate media for the organism to grow. Most
will
> grow on some kind of sheep blood agar, but some anaerobes require
additional
> nutrients found in specially made anaerobic blood agar.
>
> You will need an appropriate anaerobic chamber with the proper mix of
> hydrogen, nitrogen and carbon dioxide (absolutely no oxygen). This will go
> into an incubator at the proper temperature - usually 35 degrees C.
>
> MIC methods are too complicated to describe here. The E-Test is by far the
> easiest to set up, but may be a bit difficult to read.
>
> --
> John Gentile                            President,  Rhode Island Apple
Group
> yjgent at cox.net                      RIAG Web page:  www.wbwip.com/riag/
> "I never make mistakes, I only have unexpected learning opportunities!"
>
>
>
> > From: "Slender" <Sillyghost at lycos.com>
> > Organization: Giganews.Com - Premium News Outsourcing
> > Newsgroups: bionet.microbiology
> > Date: Fri, 24 May 2002 04:10:56 GMT
> > Subject: Microbio Techniques
> >
> > Greetings,
> >
> > I am looking for procedures to:
> >
> > 1. Isolate an anaerobic bacteria from a natural source (skin).
> > 2. Maintain the organism
> > 2. Test the sensitivity to various antibiotics.
> >
> > I've checked out Bergey's Manual and gleaned a few good sources, but I
need
> > detailed procedures. Is there a reference or publication search gateway
that
> > would assist me? Any help would be appreciated.
> >
> >
> >
> >
>
>





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