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Advice in microbiology needed

Vince Mulholland Vince.Mulholland at sasa.gsi.gov.uk
Mon May 27 05:04:24 EST 2002


Yes, it is likely to be a carcinogen. Handled with good laboratory practice
it will be quite safe to use.

Vince
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Is chloramphenicol carcinogenmic ? 


D 



"Vince Mulholland" <Vince.Mulholland at sasa.gsi.gov.uk> wrote in message 
news:C39747D75908D31195EE0090273C23E9F41B62 at barra... 
> Vladimir, 
> 
> Use chloramphenicol NOT ampicillin (the bacterium is Cam-R). This seems to

> be your problem. 
> 
> 
> 
> Vincent Mulholland, 
> Molecular Biology Unit Manager, 
> Diagnostics & Molecular Biology Section, 
> Scottish Agricultural Science Agency, 
> East Craigs, 
> Edinburgh EH12 8NJ, U.K. 
> 
> URL: http://www.sasa.gov.uk/ 
> E-Mail: Vince.Mulholland at sasa.gsi.gov.uk 
> 
> 
> 
> Hi, 
> 
> BL21 CodonPlus (DE3) RIL from Stratagene has genotype E. coli B F(-) ompT 
> hsdS(rB(-) mB(-)) dcm(+) Tet(r) gal ?(DE3) endA Hte[argU ileY leuW 
Cam(r)], 
> and appears it should be able to grow in a minimal medium. We tried to 
grow 
> it in M9 with glucose (and ampicillin for plasmid maintenance) with no 
> avail. Tech. rep. from Stratagene was not of much help suggesting to add 
> proline (?), cysteine (?) and thiamine (?) with no guarantee to work. Any 
> advice what may be a problem and how to make it grow? 
> 
> Thanks, 
> 
> Vladimir Yamshchikov 
> 
> --- 

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