In what context are you doing this? Are you in a well supplied lab or are
you doing this as a hobby? Do you know exactly what organism you are
planning on working with? Anaerobes don't respond very well to most
sensitivity testing. NCCLS standards at one time listed only broth dilution
as the standard for anaerobe sensitivities, but now the E-Test and other MIC
methods work well.
Basically you need the appropriate media for the organism to grow. Most will
grow on some kind of sheep blood agar, but some anaerobes require additional
nutrients found in specially made anaerobic blood agar.
You will need an appropriate anaerobic chamber with the proper mix of
hydrogen, nitrogen and carbon dioxide (absolutely no oxygen). This will go
into an incubator at the proper temperature - usually 35 degrees C.
MIC methods are too complicated to describe here. The E-Test is by far the
easiest to set up, but may be a bit difficult to read.
John Gentile President, Rhode Island Apple Group
yjgent at cox.net RIAG Web page: www.wbwip.com/riag/
"I never make mistakes, I only have unexpected learning opportunities!"
> From: "Slender" <Sillyghost at lycos.com>
> Organization: Giganews.Com - Premium News Outsourcing
> Newsgroups: bionet.microbiology
> Date: Fri, 24 May 2002 04:10:56 GMT
> Subject: Microbio Techniques
>> I am looking for procedures to:
>> 1. Isolate an anaerobic bacteria from a natural source (skin).
> 2. Maintain the organism
> 2. Test the sensitivity to various antibiotics.
>> I've checked out Bergey's Manual and gleaned a few good sources, but I need
> detailed procedures. Is there a reference or publication search gateway that
> would assist me? Any help would be appreciated.