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Autoclaves

lamb loes at chello.nl
Wed May 8 15:15:30 EST 2002


Lesley Robertson wrote:

> "JEDilworth" <bactitech at nospamhortonsbay.com> wrote in message
> news:3CD8890A.D7D70BD8 at nospamhortonsbay.com...
> > Is a cotton wool bung the stuff they used to use 35 years ago to stopper
> > tubed media? I haven't ever used those in a work situation - last time
> > was in college in 1970. Was this recent?
> >
> We still use cotton plugs in conical flasks for culturing aerobes, and in
> Kluyver flasks for most things.
> Lesley Robertson
> http://www.beijerinck.bt.tudelft.nl

We use cotton plugs for sterilizing conical flasks with all types of culture
media (the small batches, up to 3 liters).
To prevent sticky and charry bits of  cottonwool in the agarplates, we use one
gauze around the plug.
Loes




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