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Autoclaves

Graham Shepherd muhero.nospam at globalnet.co.uk
Thu May 2 15:14:54 EST 2002


JEDilworth <bactitech at nospamhortonsbay.com> wrote in message
news:3CD0BA49.E1E01171 at nospamhortonsbay.com...
<snip>
I wouldn't worry so much about damaging the agar as damaging
> YOU.
<snip>

If you really overcook it, you can damage the nutrients - caramelising
sugars, destroying growth factors etc - so some fastidious species won't
grow or may be killed.

<snip>

> I would think reusing microscope slides would be a pain. You'd have to
> clean them off, they stick together, and would generally be much more
> trouble than they're worth. I would buy a few boxes and be done with it.

I'm sure he doesn't need the slides sterile - steeping them in hypochlorite
solution overnight and then washing is probably sufficient. Might be enough
for the  testtubes too.

GS





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