non-radioactive way to quantify translation in E. coli

Rogier Stuger rogier666 at hotmail.com
Thu Mar 7 09:24:48 EST 2002

Hot way to quantify in vivo translation: grow cells, feed them some
hot amino acid, separate protein from unincorporated label, and count
the counts.

Anyone ever tried a non-radioactive way? Fluorescent? Sensitive enough
to quantify translation during 5 minutes of exponential growth?

Rogier Stuger
Mol Cell Physiol, Free Univ Amsterdam, NL

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