Hi folks,
I would like to insert the recognition sequence of a specific protease (e.g.
Xa or Enterokinase) in to the middle of AraC. The idea is to make an
inducible pBAD promoter that I can turn on by expressing AraC (with
arabinose of course) and then turn off by expressing the protease. I plan
to use this system in e-coli.
Can anyone recommend a good protease to use for this purpose, or give me any
tips or warnings about this idea (I am new to proteases). Has anyone done
anything like this before?
thanks a bunch for any advice you can supply,
-mike cantor
---
