Or maybe you could use spectrophotometry at 540-620nm to measure OD of the
liquid culture. I believe Acetobacter xylinum can grow in clear liquid
media.
Emir
"John Gentile" <yjgent at cox.net> wrote in message
news:B992F1AB.408D%yjgent at cox.net...
> Just thinking off the top of my head. How about:
>> 1 - set up a simple broth culture with a known amount of organism. Proof
can
> be made with a simple colony count of a measure amount spread on an agar
> plate.
>> 2 - incubate at the optimum temp (or even use different temps). and
perform
> colony counts at different intervals.
>> 3 - plot the results and calculate the growth rate and the generation
time.
>> The colony counts would have to be done on serial dilutions until you can
> get a countable number of colonies.
>> --
> John Gentile President, Rhode Island Apple
Group
>yjgent at cox.net RIAG Web page: www.wbwip.com/riag/
> "I never make mistakes, I only have unexpected learning opportunities!"
>>>> > From: gerchman at Princeton.EDU (Yoram Gerchman)
> > Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
> > Newsgroups: bionet.microbiology
> > Date: 28 Aug 2002 21:11:05 +0100
> > Subject: acetobacter xylinum growth rate?
> >
> > Greetings netters
> > does anyone know of a simple way (to be used in a high school lab) to
> > estimate growth rate of acetobacter xylinum?
> > Thanks Yoram
> > ---------------------------------------------------------
> > "Support bacteria, it's the only culture some people have."
> > Anonymous
> >
> > ---
>
