Dr. Blaschke,
I'm no expert on E.coli strains, but you raised my curiosity so I did
some searching. Here's what I found.
NB42 as an indicator strain for water quality monitoring in Sydney:
http://www.sydneywater.com.au/html/AER2000/html/qual_inland/hawkes_berowra_bact.htm
Found it in the following HTML document. Perhaps Y.Q.GOSSER etal. would
reveal a source:
HEADER KINASE 03-OCT-97 1AWO
TITLE THE SOLUTION NMR STRUCTURE OF ABL SH3 AND ITS RELATIONSHIP
TITLE 2 TO SH2 IN THE SH(32) CONSTRUCT, 20 STRUCTURES
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: ABL TYROSINE KINASE;
COMPND 3 CHAIN: NULL;
COMPND 4 FRAGMENT: SRC-HOMOLOGY 3 (SH3) DOMAIN;
COMPND 5 EC: 2.7.1.112;
COMPND 6 ENGINEERED: YES;
COMPND 7 MUTATION: N64S, N120S
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;
SOURCE 3 ORGANISM_COMMON: HUMAN;
SOURCE 4 PLASMID: PGEX;
SOURCE 5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE 6 EXPRESSION_SYSTEM_STRAIN: NB42;
SOURCE 7 EXPRESSION_SYSTEM_PLASMID: PGEX;
SOURCE 8 OTHER_DETAILS: EXPRESSED AS GST FUSIONS AND CLEAVED
KEYWDS KINASE, SH3 DOMAIN, TRANSFERASE, PHOSPHOTRANSFERASE,
KEYWDS 2 PROTO-ONCOGENE, MULTIPLE DOMAIN, LEUKEMIA
EXPDTA NMR, 20 STRUCTURES
AUTHOR D.COWBURN
REVDAT 1 28-JAN-98 1AWO 0
JRNL AUTH Y.Q.GOSSER,J.ZHENG,M.OVERDUIN,B.J.MAYER,D.COWBURN
JRNL TITL THE SOLUTION STRUCTURE OF ABL SH3, AND ITS
JRNL TITL 2 RELATIONSHIP TO SH2 IN THE SH(32) CONSTRUCT
JRNL REF STRUCTURE (LONDON) V. 3 1075 1995
JRNL REFN ASTM STRUE6 UK ISSN 0969-2126 2005
Good Luck,
Steve Stoddard
Ulrich Blaschke wrote:
>> Does anyone has informations about the E.coli strain NB42.
> A commercial source of the strain would be extremly helpful.
>> thanks, Ulrich
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