IUBio

Chemotaxis

Emir Khatipov khatipovNO at NOuchicago.edu
Thu Aug 1 15:33:14 EST 2002


I remember it is also possible to monitor taxis on microscopy slides. The
paper of Howard Gest (Check pubmed PMID: 7710317) comes to mind. I guess
textbook by Gerhardt
(http://www.amazon.com/exec/obidos/ASIN/1555810489/qid=1028233911/sr=1-3/ref
=sr_1_3/103-5407546-8967058) would also have more protocols.
Emir

"Rodrigo Arteaga" <eceh1 at hotmail.com> wrote in message
news:6eaa8267.0208010935.7b364d65 at posting.google.com...
> Hi.
>
> I am trying to perform a bacteria chemotaxis assay testing different
> substrates as chemoattractants or chemorepellents but I would like to
> know if someone knows a different or additional method than the HAP
> (hard agar plug assay) in which the bacteria respond to the gradient
> of the chemical created by it's difusion from tah agar plug into
> semisolid agar or the semisolid agar + the substrate o the capillary
> assay.
>
> Thanks in advance.
> R. Arteaga





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