Check you lab suppliers, like Gibco (Invitrogen) and others. There is a
number of mycoplasma elimination (and detection) kits on the market. I
myself liked BM-Cyclin from Roche (no affiliation).
The simplest way (but not most reliable) to detect mycoplasma is to make
microscopic cell spreads in PBS (better to fix cells before), let cells dry
and mount the slide in 50-90% glycerol with DAPI (0.5-1.5 mg/ml). You will
be able to see blue inclusions of mycoplasma DNA in the cytoplasm of the
cells. This method is not very reliable because 1) mitochondrial DNA also
binds DAPI and will look similar to mycoplasma (you need control of
non-contaminated cells), and 2) the cytoplasm layer in some trypsinized
cells is too narrow (so growing cells on slides is recommended).
I remember Roche also had a good mycoplasma detection kit.
Emir
"Ulrich" <ulrich.huth at pharmazie.uni-freiburg.de> wrote in message
news:aajnsp$q3b$1 at n.ruf.uni-freiburg.de...
> Yes, these black spots could be Mycoplasma. What should I do against this
> contamination?
> Do I have to discard my cell lines?
> Should I use some antibiotics to cure my cells?
>>>>> "Editor" <Giant_Fideli at hotmail.com> schrieb im Newsbeitrag
> news:ynly8.3321$p56.765990 at newsb.telia.net...> > Mycoplasma!
>>> > "Emir Khatipov" <khatipovNO at NOuchicago.edu> wrote in message
> > news:2Wfy8.100$s4.10280 at news.uchicago.edu...> > > Golgi, lysosomes?
>>> > > > Dear all,
> > > >
> > > > is there anybody out there working with COS-7 cells?
> > > > As I used the maximal magnification I saw black spots moving around
in
> > the
> > > > cell. First I thought, these spots are bacterias, since they did not
> > seem
> > > to
> > > > follow special pathways.
> > > > But if they are bacterias they should grow exponential, right? My
> black
> > > > spots just stay as they are.
> > > > Does anybody have an idea?
> > > >
> > > > Ulrich.
> > > >
> > > > PS: I attached the picture of my cos-7 cells. Please have a look.
>>
