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Microbial lag phases

Alex Rickard arickard at aol.com
Thu Sep 20 05:30:43 EST 2001


As defined in Microbiology (Brock, Harley and Klien)Lag phase is a
phenomenon which is associted with the inoculation and growth of cells
in batch (or related) culture. Lag phase, or the period when cells are
introduced into fresh liquid culture medium, is often related to the
type of medium used and the species of bacteria introduced into it. It
does not matter if the cells take a long period to grow or even if a
percentage become no longer viable, as long as you get exponential
growth following the lag period. This is a common phenomenon when
examining the growth characteristics of bacteria from oligotrophic
environments (ie growing freshwater bacteria in say R2A medium). All
you are essentially doing is "training" the bacteria to grow in a
different type of medium (ie selecting for a particular sub-population
in the original inoculum to grow in you defined/complex medium).

Hope this helps,


Dr. Alex Rickard
University of Manchester
School of Pharmacy

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