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lost of degradation ability

Felipe Wettstein felipe.wettstein at ioez.tu-freiberg.de
Tue Sep 11 09:45:45 EST 2001

Hi all
I isolated some strains that degrade chlorobenzene (plating on 
mineralmedium, fed with only chlorobenzene, replicaplating to prove growth).
Then I stored them in -80 C. (50% glycerol, colonies from fresh 
mineralmedium agar, fed with chlorobenzene). 
And now, something very interesting happend: When I take out the strains, 
some do not eat the dirt any more (replika plating).
Has anyone had the same experience? Does anyone has suggestions how I could 
manage to have my bacteria eating the dirt again.
thank you


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