IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Plasmid sequencing

Sylvain Kuony skuony at club-internet.fr
Thu May 31 14:55:28 EST 2001


A possible way is to cut your plasmid with an endonuclease (recognizing a
not too short sequence, at least 6 bp)
 Then if you to sequence it by yourself you can use the site of restriction
as a primer, starting point of the sequencing.
If you want it to be sequenced by a society, you can try to clone it in a
vector as pUC.

Good Luck.





More information about the Microbio mailing list

Send comments to us at biosci-help [At] net.bio.net