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Spor-Klenz and Polysorbate 80 and Lecithin

Des O'Connor des at micro-search.com
Wed May 30 02:13:02 EST 2001


Hi

You dont say what the active microbial in SPor-Klenz or what the relative
masses of avialable neutralisers are in the agar. This information would
assist in providing a usefull answer.

If you dont have that information available try running some ecomteric
controls  on  media with and with out  disinfectant loading. That way you
will prove or otherwise the effeciency of your neutralising system and such
as action is really impertaive  anyways.

You mention assesment after the antimcrobial has dried.

Would that be effective ? after all unless the active is voltaile at room
tempertaure it will leave solid antimicrobial residues after evapouration.
Such resdiues could be picked up by the Rodac plates.



Hope this helps

Des

<debra.m.guerra at abbott.com> wrote in message
news:OF27115139.2F4E09A2-ON85256A5B.0061F311 at cmis.abbott.com...
> I am a microbiologist performing a study on the effectiveness of
Spor-Klenz
> from Steris on different microorganisms on 3 different surface types.
My
> protocol calls for me to innoculate my material samples with bacteria then
> spray the Spor-Klenz on and let it act for different incubation times.  At
the
> end of the time, I am supposed to use rodac plates to sample the
> microorganisms and let it incubate for 48 hours at 35C.  However, the
rodacs
> contain TSA with Lecithin and Polysorbate 80.    My concern is that when
the
> incubation time is up and the sample is still wet with disinfectant, will
the
> PS80 and Lecithin in the plate neutralize the Spor-Klenz and stop the
> activity?  If it doesn't, then I believe my results will be skewed.  If it
> does, then i guess i have to wait until the material sample is completely
dry
> before i sample it?
>
> Any feedback is very much appreciated.
>
> Thank you.
>
> Debra M. Arcuri-Guerra
> Sr. Microbiologist
> Abbott Laboratories
>
> ---





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