Are you sure that your DNA is compatible ? What I mean is - perhaps it
is so toxic that all the transformed cells croak ?
Have you tried transforming your DNA into other bacteria, just as a
control ?
Just a thought.
A.
"R. Jayakumar" wrote:
>> hi..
> I badly need some help here. I have been trying my best to transform my
> bacillus isolate with a plasmid construct. The isolate looks like a new
> strain of Bacillus subtilis. But I need to put my construct into it. I
> have tried natural competency method, electroporation etc..and even
> triparental mating. But to no avail. The strain stubbornly refuses to
> accept the DNA> Can any good samaritan give me some ideas on how to go
> about it. I will be eternally grateful to him.
> thank you
> sincerley
> jayakumar
>> ////////////////////////////////////////////////////\\\\\\\\\\\\\\\\\\\\\\\\
> \\\\\\\\\\\\\\\\\\\\\\\\\\\
> R. Jayakumar
> Dept. of Molecular Microbiology,
> School of Biotechnology,
> Madurai Kamaraj University,
> Madurai - 625021. India.
> tel: +91-452-859141(night hours), +91-452-858471 (ask to connect to 374)
> fax:+91-452-859105
> Efax: (603)-688-4665
> Email: jakkku at mrna.tn.nic.in, (send a copy to jakku at zxmail.com ....just in
> case)
> ////////////////////////////////////////////////////\\\\\\\\\\\\\\\\\\\\\\\\
> \\\\\\\\\\\\\\\\\\\\\\\\\\\
> Science is constructed of facts like a house is of bricks, but a mere
> accumulation of facts does not make science nor does a pile of bricks a
> house
> -Henri Poincare
>> ---
--
|Dr. Artem Evdokimov Protein Engineering |
| NCI-Frederick Tel. (301)846-5401 |
| FAX (301)846-7148 |
|eudokima at mail.ncifcrf.gov |
|http://www.ncifcrf.gov/plague |
