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Extracellular enzymes from Lysobacters

Bruce A. Caldwell caldwelb at fsl.orst.edu
Mon Jul 16 15:30:59 EST 2001

What do you know about the enzyme; is it constituative, induced or
derepressed? What are your culture conditions ? Are you growing under
P-limitation or with an organic P source (e.g., glycerol-phosphate) ?

Bruce Caldwell
Department of Forest Science
Oregon State University
Corvallis OR  USA

Gay Olivier-Lilley wrote:

> I am trying to isolate a secreted alkaline phosphatase from Lysobacter
> enzymogenes.  My experience at present is that the organism produces the
> enzyme only sporadically even when it is grown under the same conditions.
> Does anyone have any suggestion from experience about what factors may be
> affecting the production of this enzyme and how I might manipulate the
> conditions to favor its production?
> Thank you for your help!
> Gay
> Gay L. Olivier-Lilley, Ph.D.
> Point Loma Nazarene University
> San Diego, CA  92106
> ---

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