I am trying to isolate a secreted alkaline phosphatase from Lysobacter
enzymogenes. My experience at present is that the organism produces the
enzyme only sporadically even when it is grown under the same conditions.
Does anyone have any suggestion from experience about what factors may be
affecting the production of this enzyme and how I might manipulate the
conditions to favor its production?
Thank you for your help!
Gay L. Olivier-Lilley, Ph.D.
Point Loma Nazarene University
San Diego, CA 92106