Many bacteria produce water-soluble pigments due to iron starvation; the
pigments are siderophores that scavenge iron. Pseudomonas sp. and
Azotobacter are but a few that do this. Azotobacter can actually be
identified based on the pigments produced, much like the Pseudomonads,
and can be isolated by using a defined nitrogen-free media like Burke's.
"Dr. Artem Evdokimov" wrote:
>> N.B. You realize, of course, that the relationship between dye
> production and the actual number of surviving bugs would depend on quite
> a few environmental factors ? Bacteria shut down metabolic mathways 'at
> will' and you would have to prove first that dye production and
> bacterial counts are in direct correlation. I am certain that thre are
> bugs that have all the desired properties, but these bugs my be
> inaccessible or reqire quaint growth mediums or be pathogenic, etc.
>> Thus, direct count of surviving cells (roughly approximated by
> colony-count) is still the most accurate method.
>> Igor wrote:
> > Cyanobacteria: Anabaena, Nostoc, Plectonema, Phormidium, even
> > Scynechococcus
> > They grow slow, but if die fast, bright-blue to deep blue phycocyanine
> > looks just great. It is water soluble
> > Ethidium wrote:
> > >
> > > Hi
> > >
> > > I'm after finding any common strains of bacteria that may produce visible
> > > water soluble pigments for a research project on bacterial growth/survival .
> > > I'm after using the pigment production as an indicator of growth/survival.
> > >
> > > So far the only bacteria I have found is pseudomonas aeruginosa ... are
> > > there any others that are common?
> |Dr. Artem Evdokimov Protein Engineering |
> | NCI-Frederick Tel. (301)846-5401 |
> | FAX (301)846-7148 |
> | eudokima at mail.ncifcrf.gov |
> | http://www.ncifcrf.gov/plague |
C. J. Fields
Graduate Student, Dept. of Biological Sciences
The University of North Texas
email : cjfields at jove.acs.unt.edu
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