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Dr. Artem Evdokimov eudokima at mail.ncifcrf.gov
Thu Jul 5 17:20:18 EST 2001

> Do you happen to know why is it that according to the handbooks agar always has to be
> dissolved by boiling before autoclaving?

Because biology is protocol-driven ? Allow me to demonstrate: a fellow
in one of the labs I have been in contact with, has been caught trying
to autoclave a huge jar of  chlorophorm. When asked why did he do it, he
pointed to the words 'autoclave everything'  a large protocol book. It
never even dawned on the dude to apply a little bit of grey matter. Who
knows what other things has he been autoclaving - the protocols called
for phenol, ethidium bromide and other wonderful substances. And, of
course, thus the mystery of why the insides of the autoclave where
turning green (copper oxide) at an alarming rate was solved.

|Dr. Artem Evdokimov   Protein Engineering |
| NCI-Frederick        Tel. (301)846-5401  |
|              FAX (301)846-7148           |
|        eudokima at mail.ncifcrf.gov         |
|      http://www.ncifcrf.gov/plague       |

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