IUBio

magnetic beads

dawe six_strings at libero.it
Wed Jan 17 17:44:14 EST 2001


Hi there! I'm trying to capture chromosomial DNA of S. bovis from a total
extract of a ruminal biofilm sapmle, in order to study the remaining DNA.
To achieve this I'm be going to use magnetic beads/streptavidin and a
biotinilated probe, this one obtained by amplifying the ITS sequence of S.
bovis (ca 380 bp).
The methods hence used don't seem to give good results...

Does anybody uses MB and would suggest me a protocol?

thanks

Davide







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