Lost of plasmids

marcini at quetzal.innsz.mx marcini at quetzal.innsz.mx
Wed Jan 17 16:39:21 EST 2001

Hi everbody.

I have a couple of cuestions:

1)Does anyone know how easily ETEC can lost his plasmids (specially ST)?
2)Which is the best storage medium that allows to keep the plasmids for a long 
term, mainly at room temperature?

I am asking this because I have been working with this bacteria since a year 
ago, and I have noted that my strains have lost his plasmids (ST). I am 
detecting the plasmid by PCR and I am worried about the time between the 
isolation and precess time (3-4 months), I have been using the Dorset egg 
medium at room temp. (is it ok?).

Thanks in advance.

Miriam Arciniega.

Instituto Nacional de la Nutricion Salvador Zubiran.
Mexico D.F. Mexico               http://www.innsz.mx


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