Hi everbody.
I have a couple of cuestions:
1)Does anyone know how easily ETEC can lost his plasmids (specially ST)?
2)Which is the best storage medium that allows to keep the plasmids for a long
term, mainly at room temperature?
I am asking this because I have been working with this bacteria since a year
ago, and I have noted that my strains have lost his plasmids (ST). I am
detecting the plasmid by PCR and I am worried about the time between the
isolation and precess time (3-4 months), I have been using the Dorset egg
medium at room temp. (is it ok?).
Thanks in advance.
Miriam Arciniega.
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Instituto Nacional de la Nutricion Salvador Zubiran.
Mexico D.F. Mexico http://www.innsz.mx
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