growth media for waking bacteria

Richard Oeffner roeffner at csusm.edu
Wed Jan 17 12:38:11 EST 2001

In my experience, you use the normal media that you use for growing the 
organism in question. I have been doin this for a year, with several 
hundered samples and have had very few problems with growth from frozen 
cultures.  The only precaution I usually take is in the freezing, by 
adding glycerol before placing samples in cryotubes and then freezing 

Richard Oeffner
Cal State San Marcos, USA

Tracy Marsh wrote:

> Does anybody have a general list or know of a resource that tells us which
> specific media to use to wake frozen bacteria, including growth conditions?
> Tracy Marsh 
> Technical Officer
> Deakin University (Rusden Campus)
> 662 Blackburn Road,
> CLAYTON, Vic.  3168
> Ph.  (03) 9244-7162
> ---

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