IUBio

growth media for waking bacteria

Colin A. B. Davidson c.davidson at biotech.cam.ac.uk
Wed Jan 17 05:05:26 EST 2001


"Tracy Marsh" <marshy at deakin.edu.au> wrote in message
news:4.1.20010117150158.00916e20 at mail-r.deakin.edu.au...
> Does anybody have a general list or know of a resource that tells us which
> specific media to use to wake frozen bacteria, including growth
conditions?
> Tracy Marsh
> Technical Officer
> Deakin University (Rusden Campus)
> 662 Blackburn Road,
> CLAYTON, Vic.  3168
>
> Ph.  (03) 9244-7162

Depending on the bugs in question, you can often just plate them straight
out onto the appropriate growth media that you normally use, without too
much extra loss of viability. With some of the sporulating bugs (e.g.
Bacillus) it's sometimes useful to heat shock them first, but again the same
growth medium as you normally use should be fine.







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