I am a final year Undergraduate at the University of Surrey, my
research project is looking at the effect of the addition of co-
metabolic substrates upon the microbial degradation of
phenanthrene.
I am having trouble with extracting the phenanthrene from the soil I am
using in order to assess its degradation using gas
chromatography. I do not have access to working Soxhlet Extraction
Apparatus nor the equipment to conduct an extraction using
Supercritical Carbon Dioxide, which seem to be the most popular
methods. Instead I have been using a simple hexane extraction
in order to try to recover the phenanthrene but this does not seem to
be effective. I have tried drying the soil samples to remove
the water content and increasing the amount of agitation the samples
undergo.
Are the any further suggestions to improve this method and/or do you
know of any other robust and yet simple methods for the
recovery of phenanthrene from soil?
Sent via Deja.com
http://www.deja.com/
