Dilworth <bactitech at hortonsbay.com> wrote:
>No one has commented on my biggest question - ARE YOU USING TUBE COAGS
>TO CONFIRM THAT THESE ARE INDEED STAPH. AUREUS?
>>I spoke to our Ph.D. microbiologist yesterday about this thread. He's
>been in the biz over 30 years in clinical laboratories. He still
>considers the tube coag as the "gold standard" test for Staph. aureus,
>irregardless of all the "new fangled" stuff out there (although he
>chuckled and alluded to the observation that one could tell the age of
>the techs doing the work by what reagents are being used, and that tube
>coags were showing our age :-) ).
>>Can anyone comment?
Our lab never bothered about the "new fangled stuff".
My age determines that, I suppose ....
We still use EDTA rabbit plasma slide coagulase.
If colony morphology doesn't fit thr result we confirm with tube coagulase.
Same plasma, diluted in broth.
I would like to use DNAse routinely as well, but there are people even older than me!
Loes