John Gentile yjgent at home.com
Thu Feb 22 21:17:59 EST 2001

It's been a while since I've used it, but I remember that it was no harder
to set up than a regular culture, but could detect Mycobacterial growth 1-2
weeks earlier than the solid media.

As I remember: Treat specimen as per usual - we used NALC with 4% NaOH for
30 minutes to digest then buffered to 6.8 pH with M15 buffer. We used the
centrifuged button to inoculate our regular tubes and a MGIT tube. The MGIT
tubes are prepared with an antibiotic mixture that is adjusted to your
contamination level (single strength for normal use, double strength in
certain situations).

The MGIT tubes are examined several times per week with a "black light" (UV
light) - and be sure to wear polarized glasses. If the tube glows bright
orange then it is positive - always keep an active culture on hand to
compare. If there is little or no color it is negative. Some bacteria, esp.
Pseudomonas can cause a false positive, but an acid fast smear of the tube
will prove it as a negative.

Our lab never did ID work so we sent out the positive MGITs to our reference
lab and they did a probe on it for a fast turn around time.

John Gentile                                    Rhode Island Apple Group
yjgent at home.com                                      President
 "I never make mistakes, I only have unexpected learning opportunities"

> From: iobrls at hotmail.com (barlas)
> Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
> Newsgroups: bionet.microbiology
> Date: 22 Feb 2001 15:42:00 -0000
> Subject: MGIT
> Would you please help us about the methodology of MGIT?
> And could you give some internet addresses how to reach sources about
> asap please....
> Yours sincerely
> iobrls at hotmail.com
> ---

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