IUBio

Simmons Citrate Agar

Bob bbruner at uclink4.berkeley.edu
Mon Feb 12 20:44:19 EST 2001


On Mon, 12 Feb 2001 08:43:13 -0000, "Colin A. B. Davidson"
<c.davidson at biotech.cam.ac.uk> wrote:

>
>"lamb" <L.A.M.Buisman at chello.nl> wrote in message
>news:3A8455BB.769FF1C4 at chello.nl...
>> Isn't that called Koser broth ?
>>
>
>Errm, I think Koser is used for the same kind of thind, but it's purely a
>growth/no growth tes (if memory serves), whereas with Simmons you get a
>colour change.
>I guess I'll just have to not be lazy and make my own up:-)

According to my old Difco manual...

Simmons citrate agar = Koser broth + bromthymol blue (80 mg/L) + 1.5%
agar. However, looking at their actual recipes shows some other small
differences.

The Manual discusses how the agar medium was devolved, in response to
problems with the broth. 

Maybe try the Koser broth + indicator?

bob







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