Simmons Citrate Agar

Bob bbruner at uclink4.berkeley.edu
Mon Feb 12 20:44:19 EST 2001

On Mon, 12 Feb 2001 08:43:13 -0000, "Colin A. B. Davidson"
<c.davidson at biotech.cam.ac.uk> wrote:

>"lamb" <L.A.M.Buisman at chello.nl> wrote in message
>news:3A8455BB.769FF1C4 at chello.nl...
>> Isn't that called Koser broth ?
>Errm, I think Koser is used for the same kind of thind, but it's purely a
>growth/no growth tes (if memory serves), whereas with Simmons you get a
>colour change.
>I guess I'll just have to not be lazy and make my own up:-)

According to my old Difco manual...

Simmons citrate agar = Koser broth + bromthymol blue (80 mg/L) + 1.5%
agar. However, looking at their actual recipes shows some other small

The Manual discusses how the agar medium was devolved, in response to
problems with the broth. 

Maybe try the Koser broth + indicator?


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