Transformation of P1 plasmid

guest01 at guest01 at
Mon Feb 12 00:34:13 EST 2001

I am hoping someone out there can help me. I am trying to electroporate
a very large P1 phage plasmid that is approximately 80kb in size into
E.coli competent cells. I have only 0.1 and 0.4 cuvettes at my disposal,
no 0.2 cuvettes but I don't  think that is the problem. Right now I am
trying different amounts of DNA and cells, and different voltages, but
with no success so far. Does anyone have any knowlege that can shed some
light on this problem? I would be very grateful.


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